Spectrophotometric kinetic studies and substrate inhibition of hog kidney histaminase activity by an improved 2,4-dinitrophenylhydrazine method.
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چکیده
منابع مشابه
Determination of Trace Amounts of Iodide by an Inhibition Kinetic Spectrophotometric Method
In this study, the reaction between Co(III)-EDTA and hypophosphite ion, catalyzed by Pd(II), was chosen as the indicator reaction. The inhibition kinetics of this catalytic reaction were investigated in the presence of iodide ion and the possibility of its analytical application was evaluated. Catalyzers other than PdCl2 (Pt, Au and Ni salts) were assayed for the indicator reaction and it was o...
متن کاملThe inhibition of histaminase by amidines.
In normal Amount Total Error of recovery Method plasma added found (%) Spectrophotometric 079 0-5 1*28 -3-4 1*0 0.4 1*41 +2-5 -1*0 1.0 1*96 -4.0 -0-82 0-7 1-53 +1*4 -0*82 1*2 2*18 -1*7 Photoelectric 076 0 5 1F24 -4 0 -1-03 0-4 1*43 +1'2 -1-03 1.0 2*0 -3*0 -0'84 0 7 1*56 +2-8 -0*84 1*2 2*01 -2.5 Roe & Kuether (1943) -0-72 0 5 1-2 -4 0 -1.0 0.4 1*4 0.0 -1.0 1.0 1*96 -4*0 -0*88 0*7 1*6 +2-8 -0*88 ...
متن کاملStudies of hog kidney acylase I. II. Some aspects of substrate specificity.
In a preceding paper (1)) evidence has been presented that two enzymes of hog kidney, dialkylfluorophosphatase (2) and amino acid Acylase I (3) are separate and distinct enzymes (4). The DFPasesl are widely distributed (5, 6) ; naturally occurring substrates are unknown although these enzymes bear some resemblances to peptidases and esterases. It was also noted that there was some correlation b...
متن کاملStudies of hog kidney acylase I. I. Comparison with hog kidney dialkylfluorophosphatase.
Hog kidney Acylase I, an enzyme which hydrolyzes N-acetylL-amino acids, has been purified approximately 40-fold by Greenstein (1). During the course of the purification of hog kidney dialkylfluorophosphatase, it was consistently found that the protein fractions containing DFPasel activity also possessed very high Acylase I activity. Inasmuch as the DFPase and Acylase I activities were found to ...
متن کاملan investigation into translation of cultural concepts by beginner and advance student using think – aloud protocols
this research aims at answering the questions about translation problems and strategies applied by translators when translating cultural concepts. in order to address this issue, qualitative and quantitative study were conducted on two groups of subjects at imam reza international university of mashhad. these two groups were assigned as beginner and advanced translation students (10 students). ...
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ژورنال
عنوان ژورنال: The Japanese Journal of Pharmacology
سال: 1989
ISSN: 0021-5198,1347-3506
DOI: 10.1254/jjp.51.107